Fastidious Anaerobe Agar w/horse blood (catalogue ref. 10062) is a ready-to-use agar plate for the cultivation and antimicrobial susceptibility testing of anaerobic bacteria. This medium is recommended by the European Committee on Antimicrobial Susceptibility Testing (EUCAST) for disk diffusion of selected rapidly growing anaerobic bacteria, i.e. Bacteroides spp., Prevotella spp., Fusobacterium necrophorum, Clostridium perfringensand Cutibacterium acnes.
FAA-HB permits excellent growth of most anaerobic bacteria and is the medium chosen by EUCAST for the development of a disk diffusion method for anaerobes.
FAA-HB plates are inoculated with a bacterial suspension in 0.85% saline from overnight cultures, adhering to the 15-15-15-minute rule (suspension used within 15 minutes, disks applied within 15 minutes of inoculation, and incubation within 15 minutes of applying the disks). The antibiotic disks are applied to the agar surface. Plates are incubated in an inverted position at 36 ± 1°C for 16-20 hours in an anaerobic atmosphere (80% N2, 10% H2, 10% CO2).
At the end of the incubation period, the inhibition zone diameters are measured and interpreted according to the current EUCAST breakpoints and QC criteria for disk diffusion of anaerobes. Refer to the current EUCAST disk diffusion reading guide for anaerobic bacteria.
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90 mm plates, 20/pack
2 months shelf life
2-8°C storage temperature