Quality control of microbial characterisation tests, culture media and antimicrobial susceptibility determinations is best accomplished using microorganisms with well-documented and stable phenotypic and genotypic characteristics. 

Bacterial and fungal strains have been selected and recommended by expert bodies, such as the International Organisation for Standardisation (ISO), EUCAST, CLSI and the European Pharmacopoeia, on the basis of their suitability for monitoring test performance and ensuring the validity of results for testing used in clinical, food, pharmaceutical, water and veterinary laboratories. Products derived from the cultures in the collections should be manufactured using the minimum number of sub-cultures, to minimise the possibility of alterations to the phenotype due to mutations. Selectrol strains are manufactured exclusively from Public Health England Culture Collections (NCTC® and NCPF®) and are first generation subcultures. They are preserved during long-term storage as freeze-dried cells in order to minimise any alterations to the phenotype caused by mutations.

 

Passages 

A Selectrol® disc provides a first generation subculture that originates from a master culture sourced from Public Health England Culture Collections. The growth from the Selectrol® disc provides a working stock for laboratory testing. It is generally accepted that no more than a total of five passages should be made from the master culture, in order to avoid genetic drift and mutant selection. Therefore, no more than four passages (fresh cultures) from the working stock should be made.

Shelf life

For most strains, Selectrol® discs are guaranteed to contain at least 106 organisms at the time of purchase; this number is sufficient to ensure that when the discs are used and stored as directed there will be viable organisms cultivable until the stated end of the shelf life, which is usually 9 months from the time the vial is first opened.

Quality Control

Selectrol® batches are tested in our UKAS accredited testing laboratory number 2496.

Selectrol® Micro-organisms:

Code Micro-organism Name Strain Designation WDCM Number No. Discs Per Vial Storage Temperature Shelf Life
MM94-10 Aspergillus brasiliensis NCPF 2275 / ATCC® 16404 00053 10 -20oC to 8oC 9 months
MM21-10 Bacillus cereus NCTC 10320 / ATCC® 9634 00001 10 -20oC to 8oC 9 months
MM86-10 Bacillus cereus NCTC 7464 / ATCC® 10876 10 -20oC to 8oC 9 months
MM86-25 Bacillus cereus NCTC 7464 / ATCC® 10877 25 -20oC to 8oC 9 months
MM29-10 Bacillus subtilis NCTC 10400 / ATCC® 6633 00003 10 -20oC to 8oC 9 months
MM29-25 Bacillus subtilis NCTC 10400 / ATCC® 6634 00003 25 -20oC to 8oC 9 months
MM44-10 Bacteroides fragilis NCTC 9343 / ATCC® 25285 10 -30oC to -15oC 6 months
MM82-10 Campylobacter jejuni NCTC 11322 / ATCC®  29428 00156 10 -30oC to -15oC 6 months
MM36-10 Campylobacter jejuni NCTC 11351 / ATCC® 33560 10 -30oC to -15oC 6 months
MM28-10 Candida albicans NCPF 3255 / ATCC® 2091 00055 10 -20oC to 8oC 9 months
MM28-25 Candida albicans NCPF 3255 / ATCC® 2092 00055 25 -20oC to 8oC 9 months
MM42-10 Candida albicans NCPF 317.509 / ATCC® 10231 00054 10 -20oC to 8oC 9 months
MM42-25 Candida albicans NCPF 317.509 / ATCC® 10232 00054 25 -20oC to 8oC 9 months
MM53-10 Candida krusei NCPF 3953 / ATCC® 6258 10 -20oC to 8oC 9 months
MM54-10 Candida parapsilosis NCPF 8334 / ATCC® 22019 10 -20oC to 8oC 9 months
MM27-10 Citrobacter freundii NCTC 9750 / ATCC®  8090 10 -20oC to 8oC 9 months
MM45-10 Clostridium perfringens NCTC 8237 / ATCC® 13124 00007 10 -30oC to -15oC 6 months
MM31-10 Clostridium sporogenes NCTC 532 / ATCC® 19404 00008 10 -30oC to -15oC 9 months
MM26-10 Enterobacter aerogenes NCTC 10006 / ATCC® 13048 00175 10 -20oC to 8oC 9 months
MM26-25 Enterobacter aerogenes NCTC 10006 / ATCC® 13049 00175 25 -20oC to 8oC 9months
MM51-10 Enterobacter Cloacae NCTC 13406 10 -20┬░C to 8┬░C 9 months
MM01-10 Enterobacter cloacae NCTC 13380 / ATCC® 23355 00082 10 -20oC to 8oC 9 months
MM17-10 Enterococcus faecalis NCTC 775 / ATCC® 19433 00009 10 -20oC to 8oC 9 months
MM17-25 Enterococcus faecalis NCTC 775 / ATCC® 19433 00009 25 -20oC to 8oC 9 months
MM18-10 Enterococcus faecalis NCTC 12697 / ATCC® 29212 00087 10 -20oC to 8oC 9 months
MM18-25 Enterococcus faecalis NCTC 12697 / ATCC® 29212 00087 25 -20oC to 8oC 9 months
MM52-10 Enterococcus faecalis NCTC 13379 / ATCC® 51299 00085 10 -20oC to 8oC 9 months
MM35-10 Enterococcus hirae NCTC13383 /ATCC® 10541 00011 10 -20oC to 8oC 9 months
MM57-10 Escherichia coli (CRE) NCTC 13476 10 -20oC to 8oC 9 months
MM02-10 Escherichia coli NCTC 12241 /  ATCC® 25922 00013 10 -20oC to 8oC 9 months
MM02-25 Escherichia coli NCTC 12241 /  ATCC® 25922 00013 25 -20oC to 8oC 9 months
MM24-10 Escherichia coli NCTC 11954 / ATCC® 35218 10 -20oC to 8oC 9 months
MM24-25 Escherichia coli NCTC 11954 / ATCC® 35218 25 -20oC to 8oC 9 months
MM33-10 Escherichia coli NCTC 10536 / ATCC® 10536 10 -20oC to 8oC 9 months
MM33-25 Escherichia coli NCTC 10536 / ATCC® 10536 25 -20oC to 8oC 9 months
MM38-10 Escherichia coli NCTC 12923 / ATCC® 8739 00012 10 -20oC to 8oC 9 months
MM63-25 Escherichia coli NCTC 12923 / ATCC® 8739 00012 25 -20oC to 8oC 9 months
MM63-10 Escherichia coli NCTC 11560 10 -20oC to 8oC 9 months
MM75-10 Escherichia coli NCTC 9001 / ATCC® 11775 00090 10 -20oC to 8oC 9 months
MM34-10 Escherichia coli (mcr-1) NCTC 13846 10 -20oC to 8oC 9 months
MM93-10 Escherichia coli O157 non-toxigenic NCTC 12900 / ATCC® 700728 00014 10 -20oC to 8oC 9 months
MM93-25 Escherichia coli O157 non-toxigenic NCTC 12900 / ATCC® 700728 00014 25 -20oC to 8oC 9 months
MM81-10 Haemophilus influenzae NCTC 12699 / ATCC® 49247 10 -30oC to -15oC 9 months
MM98-10 Haemophilus influenzae NCTC 11931 10 -30oC to -15oC 9 months
MM100-10 Haemophilus influenzae NCTC 8468 / ATCC® 9334 10 -30oC to -15oC 9 months
MM37-10 Haemophilus influenzae NCTC 12975 / ATCC® 49766 10 -30oC to -15oC 9 months
MM88-10 Raoultella planticola / Klebsiella aerogenes NCTC 9528 10 -20oC to 8oC 9 months
MM58-10 Klebsiella pneumoniae (CRE) NCTC 13438 10 -20oC to 8oC 9 months
MM55-10 Klebsiella pneumoniae (CRE) NCTC 13440 10 -20oC to 8oC 9 months
MM56-10 Klebsiella pneumoniae (CRE) NCTC 13443 10 -20oC to 8oC 9 months
MM59-10 Klebsiella pneumoniae (CRE) NCTC 13442 10 -20oC to 8oC 9 months
MM04-10 Klebsiella pneumoniae NCTC 9633 / ATCC® 13883 00097 10 -20oC to 8oC 9 months
MM04-25 Klebsiella pneumoniae NCTC 9633 / ATCC® 13883 00097 25 -20oC to 8oC 9 months
MM83-10 Klebsiella pneumoniae NCTC 13368 / ATCC® 700603 10 -20oC to 8oC 9 months
MM76-10 Lactobacillus brevis NCTC 13386 / ATCC® 8287 10 -20oC to 8oC 9 months
MM99-10 Legionella anisa NCTC 11974 / ATCC® 35292 10 -30°C to -15°C 9 months
MM92-10 Listeria innocua NCTC 11288 / ATCC® 33090 00017 10 -20oC to 8oC 9 months
MM48-10 Listeria monocytogenes NCTC 7973 / ATCC® 35152 00109 10 -20oC to 8oC 9 months
MM48-25 Listeria monocytogenes NCTC 7973 / ATCC® 35152 00109 25 -20oC to 8oC 9 months
MM77-10 Listeria monocytogenes NCTC 13372 ATCC® 7644 10 -20oC to 8oC 9 months
MM87-10 Listeria monocytogenes NCTC 11994 00019 10 -20oC to 8oC 9 months
MM47-10 Listeria monocytogenes NCTC 10527 /ATCC® 13932 00021 10 -20oC to 8oC 9 months
MM05-5 Neisseria gonorrhoeae NCTC 8375 / ATCC® 19424 5 -30oC to -15oC 4 months
MM96-5 Neisseria gonorrhoeae NCTC 12700 / ATCC® 49226 5 -30oC to -15oC 4 months
MM43-10 Proteus mirabilis NCTC 13376 / ATCC® 14153 10 -20oC to 8oC 9 months
MM68-10 Proteus mirabilis NCTC 10975 10 -20oC to 8oC 9 months
MM09-10 Proteus vulgaris NCTC 417.505 / ATCC® 13315 10 -20oC to 8oC 9 months
MM09-25 Proteus vulgaris NCTC 417.505 / ATCC® 13315 25 -20oC to 8oC 9 months
MM10-10 Pseudomonas aeruginosa NCTC 12903 / ATCC® 27853 00025 10 -20oC to 8oC 9 months
MM10-25 Pseudomonas aeruginosa NCTC 12903 / ATCC® 27853 00025 25 -20oC to 8oC 9 months
MM40-10 Pseudomonas aeruginosa NCTC 12924 / ATCC® 9027 00026 10 -20oC to 8oC 9 months
MM40-25 Pseudomonas aeruginosa NCTC 12924 / ATCC® 9027 00026 25 -20oC to 8oC 9 months
MM41-10 Pseudomonas aeruginosa NCTC 13359 / ATCC® 15442 10 -20oC to 8oC 9 months
MM65-10 Pseudomonas aeruginosa NCTC 10662 / ATCC® 25668 00114 10 -20oC to 8oC 9 months
MM97-10 Rhodococcus equi NCTC 1621 / ATCC® 6939 00028 10 -20oC to 8oC 9 months
MM73-10 Saccharomyces cerevisiae NCPF 3178 10 -20oC to 8oC 9 months
MM73-25 Saccharomyces cerevisiae NCPF 3178 25 -20oC to 8oC 9 months
MM50-10 Saccharomyces cerevisiae NCTC 10716 / ATCC® 9763 00058 10 -30oC to -15oC 9 months
MM84-10 Salmonella nottingham NCTC 7832 10 -20oC to 8oC 9 months
MM89-10 Salmonella poona NCTC 4840 10 -20oC to 8oC 9 months
MM89-25 Salmonella poona NCTC 4840 25 -20oC to 8oC 9 months
MM11-10 Salmonella typhimurium NCTC 12023/ ATCC® 14028 00031 10 -20oC to 8oC 9 months
MM11-25 Salmonella typhimurium NCTC 12023/ ATCC® 14028 00031 25 -20oC to 8oC 9 months
MM12-10 Serratia marcescens NCTC 13382 / ATCC® 8100 10 -20oC to 8oC 9 months
MM13-10 Staphylococcus aureus NCTC12981 / ATCC® 25923 00034 10 -20oC to 8oC 9 months
MM13-25 Staphylococcus aureus NCTC12981 / ATCC® 25923 00034 25 -20oC to 8oC 10 months
MM14-10 Staphylococcus aureus NCTC 12973 / ATCC® 29213 00131 10 -20oC to 8oC 9 months
MM14-25 Staphylococcus aureus NCTC 12973 / ATCC® 29213 00131 25 -20oC to 8oC 9 months
MM30-10 Staphylococcus aureus NCTC 7447 / ATCC® 6538P 00033 10 -20oC to 8oC 9 months
MM30-25 Staphylococcus aureus NCTC 7447 / ATCC® 6538P 00033 25 -20oC to 8oC 9 months
MM46-10 Staphylococcus aureus NCTC 10788 / ATCC® 6538 00032 10 -20oC to 8oC 9 months
MM46-25 Staphylococcus aureus NCTC 10788 / ATCC® 6538 00032 25 -20oC to 8oC 9 months
MM64-10 Staphylococcus aureus (MRSA) NCTC 12493 00212 10 -20oC to 8oC 9 months
MM85-10 Staphylococcus aureus NCTC 6571 / ATCC® 9144 00035 10 -20oC to 8oC 9 months
MM91-10 Staphylococcus aureus (MRSA) NCTC 13373 / ATCC® 43300 00211 10 -20oC to 8oC 9 months
MM15-10 Staphylococcus epidermidis NCTC 13360 / ATCC® 12228 00036 10 -20oC to 8oC 9 months
MM15-25 Staphylococcus epidermidis NCTC 13360 / ATCC® 12228 00036 25 -20oC to 8oC 9 months
MM16-10 Streptococcus agalactiae NCTC 8181 / ATCC® 13813 10 -20oC to 8oC 9 months
MM19-10 Streptococcus pneumoniae NCTC 12695 /ATCC® 6303 10 -20oC to 8oC 9 months
MM19-25 Streptococcus pneumoniae NCTC 12695 /ATCC® 6303 25 -20oC to 8oC 9 months
MM95-10 Streptococcus pneumoniae NCTC 12977 /ATCC® 49619 10 -20oC to 8oC 6 months
MM20-10 Streptococcus pyogenes NCTC 12696 /ATCC® 19615 10 -20oC to 8oC 9 months
MM20-25 Streptococcus pyogenes NCTC 12696 /ATCC® 19615 25 -20oC to 8oC 9 months
MM06-10 Vibrio parahaemolyticus NCTC 10885 00185 10 -30oC to -15oC 9 months
MM80-10 Yersinia enterocolitica NCTC 12982 / ATCC® 9610 00038 10 -20oC to 8oC 9 months
MM80-25 Yersinia enterocolitica NCTC 12982 / ATCC® 9610 00038 25 -20oC to 8oC 9 months

Selectrol® discs are manufactured exclusively from NCTC® (National Collection of Type Cultures) and NCPF® (National Collection of Pathogenic Fungi) cultures and are guaranteed first generation derivatives from the source strain.  ATCC® strains are listed as a reference only. ATCC® is a registered trademark of the American Type Culture Collection.